Characterization of transcriptional response for selected genes and M. bovis spoligotype composition in wild boar.

(A) Normalized S100A9 mRNA levels. (B) Normalized LTF mRNA levels. (C) Normalized PGLYRP1 mRNA levels. (D) Comparative analysis of proteomics and RT-PCR results for differentially represented immune system proteins S100A9, LTF and PGLYRP1. (E) Normalized MUT mRNA levels. (F) Normalized C3 mRNA levels. Individual RNA samples of young and adult wild boar mandibular lymph nodes (young TB-, N = 5; young TB+, N = 9; adult TB-, N = 4; adult TB+, N = 5; adult TB++, N = 5) were used for real-time RT-PCR analysis. Amplification efficiencies were normalized against S. scrofa cyclophilin, expressed as transcript copy numbers in arbitrary units and represented as average + S.D. Pair comparisons between mRNA expression levels were done by one-tailed Student’s t-test for samples with unequal variance (p = 0.05). (G) M. bovis spoligotype frequency identified in wild boar included in the study.