Characterization of the ndrg4 mutant.

<p>(A) Schematic representation of the <i>ndrg4</i> genomic locus. The extended region on the <i>exon 2</i> represents the sequence targeted by the CRISPR/Cas9 system. Red: sgRNA binding site. Blue: PAM sequence. <i>ndrg4</i><sup><i>+</i></sup> corresponds to the wild-type allele; <i>ndrg4</i> *<sup>31</sup> and <i>ndrg4</i> *<sup>34</sup> are the loss-of-function alleles used in this study. (B) Schematic of ndrg4 protein product. In <i>ndrg4</i> *<sup>31</sup> and <i>ndrg4</i> *<sup>34</sup> mutant fish, the deletions result in a frameshift generating a premature STOP codon at the level of the amino acids 31 and 34 (of 352) in the ndr family domain. Lateral views of a control (C) and a ndrg4 mutant (D) embryos at 72 hpf. The arrows point to the heart, note the pronounced heart edema (white asterisk) observed in the ndrg4 mutant. Lateral view of <i>ndrg4</i> mRNA expression in a control (E) and a ndrg4-/- embryo (F) at 48hpf. Note the absence of <i>ndrg4</i> expression in the mutant. Scale bar = 200 μm.</p>