Characterization of chimeric HPV16L1-17RG1 and 5L1-17RG1 VLP by ELISA.

(A) HPV16L1-17RG1 VLP were compared to wt HPV16 L1 VLP under native conditions using neutralizing mAb H16.V5 and H16.E70, and non-neutralizing mAb Camvir-1. (B) Serial dilutions of antisera raised against HPV16L1-17RG1 or HPV5L1-17RG1 were analyzed in triplicates by HPV17RG1 peptide ELISA. Serum titers are reported for OD values greater than the mean OD of pre-immune sera plus 3 standard deviations (SD).