Cell surface expression of CD14 and the TLR4/MD-2 complex in PLT-BMDMs.

PLT-BMDMs and control BMDMs were incubated with anti-mouse CD16/32 at 4°C for 10 min, and then incubated with biotin anti-mouse CD14 antibody or rat anti-mouse TLR4/MD-2 complex antibody at 4°C for 30 min. After incubation with streptavidin-PE or anti-rat IgG-Alexa Fluor 647 antibody at 4°C for 30 min, cells were analyzed by FACSVerse (BD Biosciences). The data are shown as histograms representing the number of cells (Y-axis) against the log of fluorescence intensity (X-axis). The black lines represent cells with primary antibody, and the gray-shaded areas represent cells without primary antibody. Experiments were repeated three times, and representative results are shown.