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Cell aggregation after pilus gene over-expression.

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posted on 2016-12-12, 18:45 authored by Mariya Tarazanova, Marke Beerthuyzen, Roland Siezen, Marcela M. Fernandez-Gutierrez, Anne de Jong, Sjoerd van der Meulen, Jan Kok, Herwig Bachmann

A—L. lactis NCDO712, B—empty vector control MG1363(pIL253), C—MG1363(pIL253pilΔ1), D—MG1363(pIL253pil). The images were taken 3 min after re-suspension of cells from an overnight culture.

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    16- kb plasmid pSH 74pilus basal subunit SpaBgram-positive model organismpilus gene clusterlactis MG 1363copper resistancestrain NCDO 712Novel Pilus Gene Cluster Lactococcus lactis MG 1363novel 8- kb pilus gene cluster spaCB-spaA-srtC 1-srtC50- kb plasmidstrain NCDO 712. Plasmid-cured strainspilus backbone protein SpaAlactis MG 1614.DNAdaughter strain MG 1363cell chainingLactococcus lactis NCDO 712pilin tip protein SpaCbasal protein spaB

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