CaMKIIα and CaMKIIβ subunits are both required for ES-induced p-CREB.

(A) Expression of CaMKIIα in cells transduced with control or CaMKIIα shRNA (mean ± SD; n = 3). β-actin was used as a loading control. (B) Expression and quantification of p-CREB in treated cells (mean ± SD; n = 3). Histone was used as loading control. *P < 0.05 compared to ES-/Control shRNA; ^#P < 0.05 compared to ES+/Control shRNA; ^&P < 0.05 compared to ES-/CaMKIIα shRNA; F-value = 24.440. (C) Expression of CaMKIIβ in cells transduced with control or CaMKIIβ shRNA (mean ± SD; n = 3). β-actin was used as a loading control. (D) Expression and quantification of p-CREB in treated cells (mean ± SD; n = 3). Histone was used as loading control. *P < 0.05 compared to ES-/Control shRNA; ^#P < 0.05 compared to ES+/Control shRNA; ^&P < 0.05 compared to ES-/CaMKIIβ shRNA; F-value = 24.173. (E) p-CREB expression in treated cells. Histone expression was used as a loading control. (F) Quantification of p-CREB treated cells (mean ± SD; n = 3). *P < 0.05 compared to sham group; ^#P < 0.05 compared to ES+; ^&P < 0.05 compared to ES+/CaMKIIα shRNA; ^$P < 0.05 compared to ES+/CaMKIIβ shRNA; F-value = 28.325.