CT induces an intact diarrheal response in mice lacking GM1 and GM1 related GSLs.
2018-02-12T18:46:33Z (GMT) by
<p><b>(A)</b> Representative histograms from flow cytometry analyses of CTB-, G33D- and OVA-binding to WT and KO jejunal cells (non-villi epithelial and CD45+ cells). <b>(B)</b> Bar graph showing CTB, G33D and OVA gMFI of non-villi jejunal epithelial cells from wt (black) and KO (gray). <b>(C-F)</b> Bar graphs showing percent of gMFI of non-blocked CTB binding to non-villi jejunal epithelial cells (wt black and KO gray) following pretreatment of the cells with <b>(C)</b> lectins or CTB with <b>(D)</b> sugars, <b>(E)</b> oligosaccharides and <b>(F)</b> HSA-linked oligosaccharides. Graphs show the percent of gMFI of CTB binding to the cells where 100% represents CTB staining with no blocking. <b>(G-H)</b> Bar graph showing intestine-animal ratio (by weight) for WT and KO mice gavaged with PBS with or without CT. <b>(H)</b> KO mice were fed chow with (open circles) or without (full circles) NB-DNJ for 4 weeks prior to gavage. The graphs are from pooled experiments where each dot represents one animal (n = 4–30). Significance was calculated using a one-way-ANOVA with Tukey correction (**** = p<0,0001, *** = p<0,005, ** = p<0,01 and * = p<0,05).</p>
CC BY 4.0