CLIC1 promotes adhesion of monocytes to and inflammation of ECs under oxidative stress.

Cont group: HUVECs with normal culture medium for 12h. H₂O₂ group: HUVECs with H₂O₂ exposure for 12h. IAA94 group: HUVECs with 40μM IAA94 pretreatment for 1 h and then H₂O₂ exposure for 12h. CLIC1^-/- group: CLIC1^-/- HUVECs with H₂O₂ exposure for 12h. (A) and (B) The pro-inflammatory cytokines IL-1β (A) and TNF-α (B) levels determined by ELISA both were increased after H₂O₂ exposure, whereas IAA94 and CLIC1 deficiency markedly obstructed their increase. (C) and (D) IAA94 and CLIC1 deficiency obviously reduced ICAM-1 and VCAM-1 relative expression levels at the protein level. *P < 0.05, **P < 0.01 versus Cont group, ^#P < 0.05, ^##P < 0.01 versus H₂O₂ group. Error bars represent SD of three replicate experiments.