CHIT1-FL crystallogenesis strategy.

(A) CHIT1-CAT crystals crushed and used for initial automated cross-seeding. (B) CHIT1-FL crystals obtained after the first cross-seeding round in crystallization condition A. (C) CHIT1-FL crystals obtained after optimization through manual hanging drop. (D) CHIT1-FL crystals from condition A crushed and used for another cycle of automated micro-seeding leading to crystallization condition F6. (E) CHIT1-FL crystals obtained after optimizing F6 condition.