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CHIT1-FL crystallogenesis strategy.

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posted on 2016-04-25, 05:30 authored by Firas Fadel, Yuguang Zhao, Alexandra Cousido-Siah, Francesc X. Ruiz, André Mitschler, Alberto Podjarny

(A) CHIT1-CAT crystals crushed and used for initial automated cross-seeding. (B) CHIT1-FL crystals obtained after the first cross-seeding round in crystallization condition A. (C) CHIT1-FL crystals obtained after optimization through manual hanging drop. (D) CHIT1-FL crystals from condition A crushed and used for another cycle of automated micro-seeding leading to crystallization condition F6. (E) CHIT1-FL crystals obtained after optimizing F6 condition.

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    house mite dust allergensCBM 14 family membersFull Length Human ChitotriosidaseHuman chitotriosidaselength CHIT 1CBM 14 domainCHIT 1ChBD CHIT 1CBM 14 familycrystallization approachchitin binding domainlatter domainAcidic Mammalian Chitinase1.95 Å resolutioncrystal structureChitin Binding Domain Chitinasesdisulfide bridgesTrp 465crystal structureschitin bindingchitinase CHIT 1cysteine residuescrystallization strategyCHIT 1 chitin binding domain

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