Fig 3.TIF (428.34 kB)
CD64 expression of CD64 on circulating neutrophils is elevated in leprosy patients with ENL.
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posted on 2016-08-24, 17:46 authored by Veronica Schmitz, Rhana Berto da Silva Prata, Mayara Garcia de Mattos Barbosa, Mayara Abud Mendes, Sheila Santos Brandão, Thaís Porto Amadeu, Luciana Silva Rodrigues, Helen Ferreira, Fabrício da Mota Ramalho Costa, Jessica Brandão dos Santos, Fabiana dos Santos Pacheco, Alice de Miranda Machado, José Augusto da Costa Nery, Mariana de Andrea Hacker, Anna Maria Sales, Roberta Olmo Pinheiro, Euzenir Nunes Sarno(A) mRNA expression of CD64 (FCGR1A) was assessed by RT-qPCR in whole blood of healthy donors (HD, n = 10), non-reactional lepromatous leprosy patients (LL, n = 8), and LL with ENL (ENL, n = 11). Box plots show median, interquartile range, sample minimum, and maximum. (B) Flow cytometry analyses of surface CD64 expression in neutrophils (PMN CD64 index) from whole blood of healthy donors (HD, n = 8), LL patients (LL, n = 8), borderline-tuberculoid leprosy (BT; n = 7), LL patients with ENL (ENL, n = 8), and reversal reaction patients (RR; n = 8). (C) Receiver operating characteristic (ROC) curves for the PMN CD64 index. Statistics: (A) ANOVA and (B) Kruskal-Wallis (* P< 0.05, ** P < 0.01 and *** P < 0.001).
