Fig 1.TIF (6.17 MB)

CD64 expression is modulated in ENL skin lesions after thalidomide- treatment.

figure

posted on 2016-08-24, 17:46 authored by Veronica Schmitz, Rhana Berto da Silva Prata, Mayara Garcia de Mattos Barbosa, Mayara Abud Mendes, Sheila Santos Brandão, Thaís Porto Amadeu, Luciana Silva Rodrigues, Helen Ferreira, Fabrício da Mota Ramalho Costa, Jessica Brandão dos Santos, Fabiana dos Santos Pacheco, Alice de Miranda Machado, José Augusto da Costa Nery, Mariana de Andrea Hacker, Anna Maria Sales, Roberta Olmo Pinheiro, Euzenir Nunes Sarno

(A) mRNA expression of CD64 (FCGR1A) was assessed by qRT-PCR in ENL skin lesions at diagnosis (ENL) and 7 days after thalidomide-treatment (ENL Thal). Each line with a symbol represents a patient (n = 10). (B) Protein levels obtained from skin lesion fragments were analyzed by Western blot using antibodies against myeloperoxidase (MPO) and GAPH (loading control). The figure shows a representative Western blot analysis of 5 patients in 2 independent experiments (n = 5). The graph shows the MPO normalized values of 5 patients. Box plots show median, interquartile range, sample minimum, and maximum indications. Biopsies of ENL (Ci and Ciii) and ENL Thal (Cii and Civ) were processed for H&E. PMNs were identified with arrowheads. Scale bars = 25 μm. (Cv) ENL and ENL Thal (Cvi) skin lesions were labelled with monoclonal antibody anti-CD64. Immunoperoxidase was performed on cryosections with haematoxylin contrast. Photomicrographs are representative sections from ENL and ENL Thal (n = 6). Scale bars: 25 μm. Statistic: (A) Wilcoxon (* P < 0.05) and (B) Student's t-test (* P< 0.05).