CB2R augments autophagy in peritoneal macrophages stimulated with LPS/DSS.

After LPS (10 ng/ml) priming for 1 h, peritoneal macrophages were stimulated with 3% DSS in the presence or absence of HU 308 (10 μM) for 24 h. In another set of experiments, peritoneal macrophages from WT and CB2R KO mice were isolated and stimulated with/without LPS/DSS for 24h. Macrophages were collected and LC3-II/LC3-I, Beclin-1 and SQSTM1 were analyzed using Western blot. (A) Pre-incubated with HU 308 significantly increased the level of LC3-II/LC3-I, Beclin-1 and decreased the level of SQSTM1. n = 6 per group. **P<0.01 vs. control, #P<0.05 vs. vehicle. (B) The expression of LC3-II/LC3-I and Beclin-1 were decreased and the expression of SQSTM1 was increased in CB2R KO group. n = 6 per group. *P<0.05 vs. control, #P<0.05 vs. WT.