Mass analysis of VesB.

VesB was overexpressed and purified from supernatants of LB-grown V. cholerae culture at ambient temperature in the absence of protease inhibitors. The purified material was subjected to intact mass analysis using an Agilent 6224 ESI-TOF mass spectrometer. Deconvoluted ESI mass spectrum indicates a major VesB species. Accuracy of the instrument: 0.01% of molecular mass.

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