AR-3-2-118-5.jpg (48.24 kB)
Blocking of monocyte migration induced by RA SCL supernatants with blocking anti-chemokine or chemokine receptor mAbs
figure
posted on 2011-12-30, 22:03 authored by Kenji Hayashida, Toshihiro Nanki, Hermann Girschick, Sule Yavuz, Takahiro Ochi, Peter E LipskyCopyright information:
Taken from "Synovial stromal cells from rheumatoid arthritis patients attract monocytes by producing MCP-1 and IL-8"
Arthritis Research 2001;3(2):118-126.
Published online 5 Jan 2001
PMCID:PMC17828.
Copyright © 2001 BioMed Central Ltd on behalf of the copyright holder
Monocyte migration induced by supernatants of RA SCLs (RA6/1 and RA8/3) was assessed using mAbs, which can neutralize specific chemokines or chemokine receptors. Concentrations of mAbs were 10 μg/ml for MCP-1, IL-8, and IP-10, and 50 μg for anti-CCR5 mAb with 5 × 10 SRBC rosette negative cells. Migrating cells were stained with anti-CD-14 mAb, and migrating CD14cells were counted by flow cytometry. The mean and SEM were calculated from the results of nine independent experiments. The paired Student test was used to assess statistical differences versus the result with control mAb.