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Biosynthesis of the Novel Macrolide Antibiotic Anthracimycin
journal contribution
posted on 2015-11-20, 11:34 authored by Silke Alt, Barrie WilkinsonWe
report the identification of the biosynthetic gene cluster for
the unusual antibiotic anthracimycin (atc) from the
marine derived producer strain Streptomyces sp. T676 isolated off St. John’s Island, Singapore. The 53 253
bps atc locus includes a trans-acyltransferase
(trans-AT) polyketide synthase (PKS), and heterologous
expression in Streptomyces coelicolor resulted in anthracimycin production. Analysis of the atc cluster revealed that anthracimycin is likely generated by four
PKS gene products AtcC–AtcF without involvement of post-PKS tailoring enzymes, and a biosynthetic pathway is
proposed. The availability of the atc cluster provides
a basis for investigating the biosynthesis of anthracimycin and its
subsequent bioengineering to provide novel analogues with improved
pharmacological properties.