BTA1 is responsible for DGTS production.

(A), (D) Thin layer chromatography (TLC) of lipid extracts from F. graminearum or E. coli expressing F. graminearum BTA1. (B), (E) Spots scraped from TLC plates (indicated by arrows) were applied to accurate-mass quantitative time-of-flight liquid chromatography mass spectrometry (Q-TOF LC MS) analysis. See Figure I in S1 Text for more lipid analyses. (C) RT-PCR results for BTA1 expression in various F. graminearum strains and conditions. (F) Subcellular localization of BTA1-mRFP in F. graminearum. ER: endoplasmic reticulum localized protein marker. White bar = 100 μm. (G) Virulence assays on maize stalks. Lesions were measured at 7 days after inoculation. Different letters indicate statistical differences among the strains according to Tukey’s multiple comparison test (P = 0.05), n = 3 independent experiments.