BDNF localizes in the injured IAN.

Histological analysis of inferior alveolar nerve (IAN) injury experiments in rats after 14 days. Azan staining (A, B) and immunohistochemical stainings of BDNF (C-G, I) and trkB (H, J). (A, C, E, G, H) Unaffected region of IAN injury. (B, D, F, I, J) Regenerating IAN region after injury. Although unaffected IAN and peripheral bone tissues did not express BDNF (C, E, G), injured IAN (D, F) and active osteoblasts on the surface of newly formed bone (I) were immunopositive for BDNF. The osteoblast lineage cells surrounding newly formed bone were immunopositive for trkB (J). IAN; inferior alveolar nerve. Bars, 100 μm (A-F), 20 μm (G-J).