Example video for raw, straightened, and tracked data.

Left: Raw video feed from high magnification RFP video. The imaging plane is scanning up and down through the volume of the worm’s brain. The recording is shown at 1/2× speed and the time elapsed is indicated in the bottom left. Middle: Maximum intensity projection of each volume is shown after Worm Centerline Tracking and Straightening. Right: Locations of neurons are shown at the end of the pipeline (after Neuron Registration Vector Encoding, Clustering and Error Correction). Each color represents a different tracked neuron. All neurons from the volume are shown overlaid on a raw image of the middle plane of each volume. Note a light flash used for time synchronization is visible around t = 13s.

(MP4)