Attenuation of riboflavin-induced resistance in response to <i>Pst</i> DC3000 in the <i>mpk3</i> and <i>mpk6</i> mutant.

2016-04-07T05:21:30Z (GMT) by Shengjun Nie Huilian Xu
<p>(<b>a</b>) Expression of <i>PR1</i> gene is compromised in the <i>mpk3</i> and <i>mpk6</i> mutant seedlings. 2-week-old WT and single mutant (<i>mpk3</i>, <i>mpk6-2</i> and <i>mpk6-3</i>) seedlings were treated with water or riboflavin, and then inoculated with <i>Pst</i> DC3000 for 24 hours. The total RNAs were extracted at indicated times and analyzed for the expression of <i>PR1</i> gene. <i>Actin2</i> was used as an internal control. Control represents WT and single mutant seedlings were not treated with riboflavin and <i>Pst</i> DC3000. Different letters indicate statistically significant differences among the samples (P <0.05; Duncan’s multiple range tests). (<b>b</b>) Representative water- or riboflavin-treated leaves at 5 days after inoculation with <i>Pst</i> DC3000. The <i>mpk3</i> and <i>mpk6</i> mutants were significantly more susceptible to <i>Pst</i> DC3000 and failed to develop resistance by riboflavin. (<b>c</b>) Pathogen growth in WT and single mutant seedlings. Different letters indicate statistically significant differences among the samples (P <0.05; Duncan’s multiple range tests). The experiment was repeated three times with similar results.</p>