Attenuation of LPS-induced NO production from BMDMs by PLT-sup.

BMDMs (2.5 × 10⁶ cells) were cultured for 24 h with PLT-sup (PLT-BMDMs) or 0.5 U/mL thrombin alone (control BMDMs) in a 3.5 cm dish, and stimulated with complete medium containing LPS (50 ng/mL) for 0–24 h. The production levels of NO₂^- in the culture supernatant were determined by Griess reaction using a calibration curve for NaNO₂. Experiments were performed in quintuplicate and repeated four times. Data are presented as the mean ± SEM. *p < 0.05, **p < 0.01 vs. control BMDMs. Representative results of the four experiments are shown.