Association of cytokine levels with foreskin neutrophil and dendritic cell density.

Immunofluorescence was used to measure neutrophil and dendritic cell densities in foreskin tissues from men with either high (n = 5) or low (n = 5) coronal sulcus IL-8 and MIG levels. A-C: Representative images of: (A) CD15+ neutrophils (green); (B) CD11c+ dermal dendritic cells (red); and (C) CD207+ Langerhans cells (green). Nuclei are counterstained with DAPI (blue) in all images. D: Median and range of foreskin neutrophil density in total foreskin tissue, Langerhans density in epidermal tissue, and dermal dendritic cell density in dermal tissue, from men with high (hatched bars) or low (open bars) cytokine levels. Groups compared using Mann-Whitney U test.