Application of the proposed method to the EFTEM P-maps.
(A-E) The results of the method application to a cyanobacterium Nostoc sp. PCC 7118 from the P-sufficient stationary phase culture. (F-J) The results of the method application to a eukaryotic microalga Chlorella vulgaris IPPAS C-1 from the P-sufficient stationary phase culture. (A) and (F) Elastically filtered TEM images of cell sections. (B) and (G) EFTEM P-maps of the cell sections. (D) and (I) Averaged profiles of the EFTEM P-maps. (C) and (H) The EFTEM maps processed via the workflow “B” (see text and Fig 1). (E) and (J) The relative entropy analysis of the EFTEM maps (B) and (G), respectively. The averaged profiles were recorded along the white lines (see the P-maps). The red outline on the processed maps (C) and (H) indicates the region taken for the inclusion area measurements. In the graphs (E) and (J) the threshold pixels g1 used for the EFTEM maps processing are designated (for details see text). PRI P-rich inclusion, PHB poly-(R)-3-hydroxybutyrate granules, T thylakoid(s), Ch chloroplast, N nucleus, S starch. Scale bars = 0.5 μm.
