1471-2148-7-39-5.jpg (36.92 kB)

And histone acetylation and transcription in African apes and humans

figure

posted on 2011-12-30, 17:10 authored by Beatrice Bodega, Maria Francesca Cardone, Stefan Müller, Michaela Neusser, Francesca Orzan, Elena Rossi, Elena Battaglioli, Anna Marozzi, Paola Riva, Mariano Rocchi, Raffaella Meneveri, Enrico Ginelli

Copyright information:

Taken from "Evolutionary genomic remodelling of the human 4q subtelomere (4q35.2)"

BMC Evolutionary Biology 2007;7():39-39.

Published online 14 Mar 2007

PMCID:PMC1852401.

Chromatin immunoprecipitation experiments on gorilla and chimpanzee lymphoblastoid cell lines before (GGO and PTR) and after Trichostatin A treatment (GGO-TSA and PTR-TSA). Chromatin was immunoprecipitated with a rabbit antiserum anti-acetyl histone H4, and a normal rabbit IgG as pre-immune (Upstate Biotechnology); the and promoter regions were amplified on Input DNA, pre-immune and H4Ac-immunoprecipitated samples using the primer pairs shown in Additional file . Quantification by real-time RT-PCR of the mRNA of the and genes; total RNA from untreated (control) and TSA-treated (TSA) GGO, PTR and HSA fibroblast cell lines was used as a template with primer pairs for and mRNAs. Each experiment was performed in triplicate (bars show SD).