Analysis of ALA formation after infecting gerbils with various sub-clones derived from clone B8^np and clone B2^p.

Clones B8^np and B2^p were sub-cloned by a limited dilution method, resulting in five sub-clones each (B8_1–5 and B2_1–5, respectively). Gerbils were infected with 1 × 10⁶ trophozoites of the various sub-clones. Seven days post infection, liver abscess formation was analysed, and area of ALA (mm²) was determined. Significance (p-values) was established by the Mann-Whitney U test. p-values were calculated relative to control (clone B8^np or clone B2^p, respectively). For detailed information see S1 Table.