An expanded GlCHC-interactome.

(A-L) IFA validation of HA-tagged candidate proteins obtained by reverse coIP: All reporter constructs localize, in varying degrees, to the cell cortex. Aside from 9 H and K, all reporters show deposition at the barezone, an indicator of PV-associated localization. Cells were imaged at maximum width, where nuclei and the bare-zone are at maximum diameter. Representative images for HA-tagged Gl17060(A), Gl17037(B), Gl6687(C), Gl7188(D), Gl7588(E), Gl10358(F), Gl13922(G), Gl16575(H), Gl17332(I), Gl21942(J), Gl92223(K) and, Gl101278(L). (M) Expanded GlCHC-interactome including IFA-validated HA-tagged proteins from A-L (spheres in light green). Full and dashed lines indicate high and low interaction stringency parameters, respectively.