Alternation of ten-eleven translocation 1, 2, and 3 expression in eutopic endometrium of women with endometriosis-associated infertility

Little is known about the differences in ten-eleven translocation 1, 2, and 3 (TET1-3) expression in the endometrial phases in eutopic endometrium from infertile women with endometriosis (IWE) and fertile women without endometriosis (FW). Using RT-qPCR and western blot analysis, we assessed the TET expression in the mid-follicular and mid-luteal phases in eutopic endometrium from IWE (n = 38) and FW (n = 18). Both IWE and FW underwent laparoscopic and histological examinations for endometriosis. In the mid-luteal eutopic endometrium in IWE, compared to that of FW, we found significantly reduced levels of TET1 transcripts and proteins (p = .001 and p = .003, respectively) at the severity stage of I/II (p = .029 and p = .003, respectively) and transcripts only at the severity stage of III/IV (p = .003). In the mid-follicular eutopic endometrium of IWE, compared to that of FW, there was a statistically significant reduction in TET2 transcript levels at the severity stage of III/IV (p = .037). Compared to the mid-follicular endometrium, we found a statistically significant increase in TET3 transcript levels during the mid-luteal phase in the eutopic endometrium of all IWE (p = .034) and in the severity stage of III/IV (p = .025). We observed a change in the expression levels of TET1-3 in the eutopic endometrium of IWE.

我们对有子宫内膜异位症的不孕妇女（IWE）和无子宫内膜异位症的非不孕妇女之间的在位子宫内膜中TET1、2、3表达的差异几无所知。我们用RT-qPCR和免疫印迹分析方法, 评估了IWE（n=38）和FW（n=18）妇女的卵泡中期和黄体中期在位子宫内膜中TET的表达情况。

IWE和FW两组妇女都通过腹腔镜手术和组织学检查对子宫内膜异位症进行了评价。我们发现与FW妇女黄体中期的在位子宫内膜相比, I/II期IWE妇女的黄体中期在位子宫内膜中TET1转录物和蛋白质的水平都显着降低（分别为p=0.001和p=0.003）、（分别为p=0.029和p=0.003）, III/IV期IWE妇女的黄体中期在位子宫内膜中只有TET1转录物的水平显着降低（p=0.003）。IWE妇女与FW妇女的卵泡中期在位子宫内膜进行比较, 在III/IV期的IWE妇女中, TET2转录物的水平有明显降低, 差异有统计学意义（p=0.037）。我们发现与卵泡中期在位子宫内膜相比, 在所有IWE妇女以及在III/IV期的IWE妇女中黄体中期的在位子宫内膜中TET3转录物水平有显着增加, 差异有统计学意义（p=0.034）和（p=0.025）。我们观察到了IWE妇女的在位子宫内膜中TET1-3表达水平的变化。