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Alkaline Phosphatase-Mimicking Peptide Nanofibers for Osteogenic Differentiation
journal contribution
posted on 2015-07-13, 00:00 authored by Gulcihan Gulseren, I. Ceren Yasa, Oya Ustahuseyin, E. Deniz Tekin, Ayse B. Tekinay, Mustafa O. GulerRecognition of molecules and regulation
of extracellular matrix
synthesis are some of the functions of enzymes in addition to their
catalytic activity. While a diverse array of enzyme-like materials
have been developed, these efforts have largely been confined to the
imitation of the chemical structure and catalytic activity of the
enzymes, and it is unclear whether enzyme-mimetic molecules can also
be used to replicate the matrix-regulatory roles ordinarily performed
by natural enzymes. Self-assembled peptide nanofibers can provide
multifunctional enzyme-mimetic properties, as the active sequences
of the target enzymes can be directly incorporated into the peptides.
Here, we report enhanced bone regeneration efficiency through peptide
nanofibers carrying both catalytic and matrix-regulatory functions
of alkaline phosphatase, a versatile enzyme that plays a critical
role in bone formation by regulating phosphate homeostasis and calcifiable
bone matrix formation. Histidine presenting peptide nanostructures
were developed to function as phosphatases. These molecules are able
to catalyze phosphate hydrolysis and serve as bone-like nodule inducing
scaffolds. Alkaline phosphatase-like peptide nanofibers enabled osteogenesis
for both osteoblast-like and mesenchymal cell lines.
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Keywords
phosphatasemesenchymal cell linescatalyze phosphate hydrolysisAlkalinecalcifiable bone matrix formationroletarget enzymesbone regeneration efficiencyphosphate homeostasisfunctionOsteogenic DifferentiationRecognitionpeptide nanofibersmoleculepeptide nanostructuresbone formationchemical structureextracellular matrix synthesis
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