1742-4690-5-11-4.jpg (55.74 kB)
Akt inhibitors as an HIV-1 infected macrophage-specific anti-viral therapy-3
figure
posted on 2011-12-31, 14:33 authored by Pauline Chugh, Birgit Bradel-Tretheway, Carlos MR Monteiro-Filho, Vicente Planelles, Sanjay B Maggirwar, Stephen Dewhurst, Baek KimRease cell survival by preventing PTEN from binding to p53. Binding of HIV-1 Tat to p53 may result in reduced levels of both p53 by destabilization and PTEN by downregulation of PTEN expression. In vitro binding assay: Lysates containing p53-FLAG were incubated with BSA (control) or HIV-1 Tat protein and then mixed with lysate containing PTEN V5-tag. Proteins bound to p53 were immunoprecipitated using anti-FLAG immobilized antibody and analyzed for PTEN-V5 tag levels by Western blotting. Ratios of PTEN-V5 levels normalized by p53-FLAG levels are shown.
Copyright information:
Taken from "Akt inhibitors as an HIV-1 infected macrophage-specific anti-viral therapy"
http://www.retrovirology.com/content/5/1/11
Retrovirology 2008;5():11-11.
Published online 31 Jan 2008
PMCID:PMC2265748.
History
Usage metrics
Categories
No categories selectedLicence
Exports
RefWorks
BibTeX
Ref. manager
Endnote
DataCite
NLM
DC