Agarose gel electrophoresis of amplified ITS1 regions of standard reference strains.

<p>H<sub>2</sub>O (lane 1) <i>L</i>. <i>major</i> (lane 2) and <i>L</i>. <i>tropica</i> (lane 3) and from CL patients (lanes 4–9) in panel A and restriction endonuclease <i>Hae</i>III digestion is shown in panel B. In both panels, MW representative of 100-bp ladder as molecular size marker. Patients in samples 2, 3, 4, 6, 7 and 9 are infected with <i>L</i>. <i>tropica</i> and patients in samples 1, 5 and 8 are infected with <i>L</i>. <i>major</i>.</p>