Agarose gel electrophoresis of amplified ITS1 regions of standard reference strains.

H2O (lane 1) L. major (lane 2) and L. tropica (lane 3) and from CL patients (lanes 4–9) in panel A and restriction endonuclease HaeIII digestion is shown in panel B. In both panels, MW representative of 100-bp ladder as molecular size marker. Patients in samples 2, 3, 4, 6, 7 and 9 are infected with L. tropica and patients in samples 1, 5 and 8 are infected with L. major.