Affinity gel electrophoresis (AGE) of OsttaDSPwild-type (WT) and C162S mutated protein (C162S).

OsttaDSP WT (WT, 5 μg) and OsttaDSP C162S (C162S, 5 μg) proteins were run on native PAGE without or with amylopectin (0.3 mg/ml) simultaneously under the same conditions. Gels were then revealed by Coomassie Blue staining. The number indicates the molecular mass marker in kDa. MMM, molecular mass markers (Amersham High Molecular Weight Calibration Kit (range 66–669 kDa)).