Additional file 9: of RNA-sequence data normalization through in silico prediction of reference genes: the bacterial response to DNA damage as case study

Contribution of in silico predictions and quadratic correction. Shown are the sample groupings for default parameters, as used in our experiment (a); grouping using a linear fit (b); and no predictions and quadratic fit (c). Out of these, only the combination of predictions and quadratic fit achieve the correct grouping. Also shown are the results when supplying a list of six differently expressed (DE) genes as reference genes (d), in which case five genes are rejected; six randomly selected genes also resolve the grouping correctly (e). (TIFF 1271 kb)