Additional file 9: Figure S8. of Imaging the mammary gland and mammary tumours in 3D: optical tissue clearing and immunofluorescence methods

Three-dimensional confocal images of mammary glands cleared using a modified CUBIC clearing protocol (Reagent 1A) to minimise protein loss. Images show the maximum intensity projection of the entire image sequence. Bottom right panel shows depth-coding of SMA-expressing myoepithelial cells in lactating mammary tissue; images in an image stack are assigned a colour based on their relative depth. Arrowhead shows non-specific intraluminal staining occasionally observed with modified CUBIC clearing, which may be improved with further washing. These images are representative of images from two mice. BV blood vessel (SMA-expressing). See Additional file 18 for a high resolution version of these PDFs. (PDF 12 mb)