Additional file 9: Figure S4. of Understanding the response to endurance exercise using a systems biology approach: combining blood metabolomics, transcriptomics and miRNomics in horses

Overview of the data analysis. Step 1: a linear model analysis of DEGs and DEmiRNAs and a supervised analysis of metabolites. Step 2: determination of the corresponding gene ontology (GO) terms, Kyoto encyclopedia of genes and genomes (KEGG) pathways and TFs and miRNAs regulating the DEGs. Step 3: selection of metabolic target genes involved in the pathways that metabolites participate in. Step 4: generation of the correlation matrix for DEGs and metabolites (in the hypergeometric test only). Step 5: the enrichment test (using the hypergeometric test or its generalization) used to select candidate-enriched metabolites. Step 6: the functional regulatory network analysis (TFs and miRNAs); Step 7: validation of the regulatory network, using an independent cohort and multiple factor analysis. (TIF 8893 kb)