Additional file 8: Figure S8. of A T-type channel-calmodulin complex triggers αCaMKII activation

Cav3-mediated activation of αCaMKII. Bar plots of the mean fluorescence intensity of pαCaMKII in cultured hippocampal cells in low [K]o (1 mM) and following exposure to 10 min of high [K]o (50 mM) prior to fixation within 10 min of the end of high [K]o exposure. Calcium influx is restricted to Cav3 channels by maintaining cells in 30 μM Cd2+, 10 μM DNQX, 100 μM DL-AP5, and 1 μM TTX. Labeling for pαCaMKII in the cytoplasm increases in high [K]o that is reduced by a peptide inhibitor specific for pαCaMKII (AIP 10 μM). Fluorescence intensity was quantified in ImageTrak software (see Methods). Values are mean ± SEM derived from n = 3–4 cover slips from at least 3 separate experiments with 29 ROIs. ** p < 0.01. (PDF 290 kb)