Additional file 7: of Chronic adiponectin deficiency leads to Alzheimer’s disease-like cognitive impairments and pathologies through AMPK inactivation and cerebral insulin resistance in aged mice

Cerebral insulin injection to the right hippocampus. (a) 2 μl of insulin (0.05I.U) or artificial cerebrospinal fluid were injected to the right hippocampus (coordination: 2.5 mm [D-V]; 2.2 mm [A-P]; 2.1 mm [Lateral] from Bregma) of 12-mth-old mice. (b) Trypan Blue dye was injected to the same coordination of the right hippocampus indicated successful injection. (JPG 35 kb)