Additional file 7: Fig. S7. of Comparative analyses of CTCF and BORIS occupancies uncover two distinct classes of CTCF binding genomic regions

Epigenetic profile of two classes of CTCF binding regions in BORIS-positive cells (K562). a Heatmaps demonstrate the association of CTCF&BORIS bound regions with active promoters (H3K4me3 and RNAPII, ENCODE) and enhancers (H3K27ac, ENCODE) mapped in K562 cells. The tag density was subjected to k-means ranked clustering with five clusters expected. b Genome browser view of two examples of Super Enhancers mapped in K562 cells. H3K27ac and p300 tracks were adopted from ENCODE. c Scatter plots show the overlapping of CTCF-only and CTCF&BORIS bound regions (left panel) and BORIS-only and CTCF&BORIS bound regions (right panel) with ChIP-seq data available for K562 cells by ENCODE. The dots represent the percentage of CTCF-only, BORIS-only (x-axis), and CTCF&BORIS (y-axis) bound regions overlapping with each factor mapped by ChIP-seq in K562 cells. The red dots are labeled with the name of the factor mapped by ChIP-seq. d The percentage of BORIS-only, CTCF&BORIS and CTCF-only bound regions (y-axis) overlapping with each factor (x-axis). e Heatmap demonstrates the enrichment of RNAPII, CAGEs, H3K4me3, H2AZ, H3K27ac, ZNF143, and SMC3 ChIP-seq data (ENCODE data) at CTCF&BORIS bound regions in contrast to CTCF-only bound regions. The tag density of ChIP-seq data was collected within a 10-kb window around the summit of CTCF peaks mapped in K562 cells. The collected data were subjected to k-means clustering using linear normalization. f Average tag density (tags/10 million) of multiple factors mapped by ChIP-seq in K562 cells (ENCODE data) across BORIS-only (blue), CTCF-only (red) and CTCF&BORIS (purple) bound regions mapped in K562 cells. The names of factors used in ChIP-seq are labeled on the top of the each plot. g Histogram shows the genomic distribution of CTCF and BORIS occupancy at promoters plus the 5’ UTR (3 kb up- and downstream of TSSs), gene bodies, and intergenic regions. (PPTX 680 kb)