Additional file 6: of Cigarette smoke alters the ability of human dendritic cells to promote anti-Streptococcus pneumoniae Th17 response

Treatment with the anti-oxidant N-acetylcystein (NAC) did not reverse the inhibitory effect of cigarette smoke extract (CSE) on the phenotype of monocyte-derived dendritic cells (MDDC) activated by S.pneumoniae (Sp). The expression of HLA-DR (a), CD80 (b), CD86 (c), CD40 (d) and CD54 (e) by MDDC treated or not with NAC and then exposed to CSE and Sp for 24 h were evaluated by flow cytometry. Data are reported as mean ± S.E.M. of 6 experiments. (PDF 39 kb)