12931_2016_408_MOESM6_ESM.pdf (39.59 kB)
Additional file 6: of Cigarette smoke alters the ability of human dendritic cells to promote anti-Streptococcus pneumoniae Th17 response
journal contribution
posted on 2016-07-26, 05:00 authored by Olivier Le Rouzic, Bachirou Koné, Jerome Kluza, Philippe Marchetti, Florence Hennegrave, Cécile Olivier, Gwenola Kervoaze, Eva Vilain, Clémence Mordacq, Nicolas Just, Thierry Perez, Nathalie Bautin, Muriel Pichavant, Philippe GossetTreatment with the anti-oxidant N-acetylcystein (NAC) did not reverse the inhibitory effect of cigarette smoke extract (CSE) on the phenotype of monocyte-derived dendritic cells (MDDC) activated by S.pneumoniae (Sp). The expression of HLA-DR (a), CD80 (b), CD86 (c), CD40 (d) and CD54 (e) by MDDC treated or not with NAC and then exposed to CSE and Sp for 24 h were evaluated by flow cytometry. Data are reported as mean ± S.E.M. of 6 experiments. (PDF 39 kb)
