Additional file 6: Figure S6. of A T-type channel-calmodulin complex triggers αCaMKII activation

Selective block of Cav1 L-type calcium channels by external Cd2+. a, b. Shown are representative recordings of calcium current evoked in tsA-201 cells expressing either the Cav1.2 or Cav1.3 calcium channel isoforms (a) or Cav3.1 calcium channels (b), with associated mean I-V plots shown below. Perfusing 30 μM Cd2+ blocks both Cav1.2 and Cav1.3 channel isoforms (a) but not Cav3.1 (b). For CaV1.2 channel expression included 2 μg each of human- α1C-PMT2, α2δ1-PMT2 and β1B-PMT2. For CaV1.3 channel expression 2 μg each of human- α1D-GFP37−, α2δ1-pcDNA and β1B-pcDNA was used. CaV1.2 and CaV1.3 expressing cells were co-transfected with 100 ng eGFPN1 for identification of transfected fluorescence cells. Average values are mean ± SEM. (PDF 572 kb)