Additional file 6: Figure S4. of Functional integration of the circulatory, immune, and respiratory systems in mosquito larvae: pathogen killing in the hemocyte-rich tracheal tufts

2016-09-19T05:00:00Z (GMT) by Garrett League Julián Hillyer
E. coli injected into larvae and adults aggregate in regions of high hemolymph flow and hemocyte concentration. (A–F) Dissected larval (A–C) and adult (D–F) dorsal abdomens with fluorescently labeled hemocytes (CM-DiI; red) at 4 h after injection with GFP-E. coli (green). In larvae, E. coli preferentially aggregated in the eighth abdominal segment (A), where there is a high concentration of hemocytes (B, circles in C). In adults, E. coli aggregated at the periostial regions of the heart (D), where the periostial hemocytes are located (E, arrows in F). (G–L) Dissected larval (G–I) and adult (J–L) dorsal abdomens with fluorescently labeled hemocytes at 24 h after injection with GFP-E. coli. The aggregation pattern of E. coli in adults at 24 h after treatment was similar to that observed at 4 h post-treatment, but in larvae, fluorescence from E. coli was not observed anywhere in the body because the infection had been largely cleared. Diagonal lines in panels G–I denote the edges of rotated images. Directional arrows: A anterior, P posterior, L lateral. (PDF 679 kb)