Additional file 5: of Genetic variants in the upstream region of activin receptor IIA are associated with female fertility in Japanese Black cattle

Transfection and co-transfection efficiencies of LβT2 cells. (A) To examine the transfection efficiency of LβT2 cells, we used the pCAGGS-EGFP and pCAGGS-mCherry vectors. At 24 h post-transfection, double fluorescence-positive cells were counted using ImageJ software. The data shown represent the transfection efficiencies of LβT2 cells in 5 experiments. The average transfection efficiency was 8.8 ± 1.62 % (3,368 cells). (B–D) To examine co-transfection efficiency in LβT2 cells, we used the pCAGGS-EGFP and pCAGGS-mCherry vectors. At 24 h post-transfection, double fluorescence-positive cells (B, magenta) were counted using ImageJ software. All GFP positive cells (C, green) were mCherry-positive (D, red), representing 100 % of 3,368 cells. (PPTX 6646 kb)