Additional file 5: Figure S4. of Sox9 transcriptionally regulates Wnt signaling in intestinal epithelial stem cells in hypomethylated crypts in the diabetic state

Sox9 acts as a transcriptional regulator in Wnt signaling. (a and b) Expression of Sox9 binding site-related genes in crypts (a) and IESCs (b). (c, e, and g) Binding profiles and peak calling records of representative Wnt component genes in Sox9 ChIP-seq analysis. Red arrows indicate peak signals. (d, f, and h) mRNA levels of Sox9 binding site-related genes after knockdown and overexpression of SOX9 in cultured cells. mRNA levels are expressed relative to β-actin. Mean ± SE; n ≥ 4; *P < 0.05, **P < 0.01, ***P < 0.001 by t test. N.S. not significant. (TIF 4817 kb)