Additional file 4: of Purification of nanogram-range immunoprecipitated DNA in ChIP-seq application

DNA profiles in H3K4me3 and H3K27me3 ChIP-seq libraries generated through different purification reagents. After library amplification with 12 cycles of PCR, DNAs were analyzed by the Fragment Analyzer. Lane 1 (EDL St), the library from 1 ng of purified ChIP DNA from aliquot A; Lane 2 (1 ng QmM), the library from stored DNA in MaxyClear tube after purification by MinElute PCR Purification Kit from aliquot B; Lane 3 (1 ng QmP), the library from stored DNA in Premium Tube after purification by MinElute PCR Purification Kit from aliquot B; Lane 4 (Input), the library from 1 ng of purified input DNA from the aliquot A. Left panel is the profile of H3K4me3 libraries, and right panel is the profile of H3K27me3 libraries. (PDF 123 kb)