Additional file 4: of Long non-coding RNA NEAT1-modulated abnormal lipolysis via ATGL drives hepatocellular carcinoma proliferation

Figure S2. ATGL promotes HCC cell growth in vitro. A. Growth curves for the indicated HCC cells were evaluated by the Trypan blue dye exclusion method (left panel). CCK-8 assays showed that overexpression of ATGL promoted the growth of HepG2 cells (right panel). B. Representative images of the cloning formation assay showed that overexpression of ATGL promoted the growth of HepG2 cells. C. Number of colonies from three experiments were measured, and the results are presented as a bar graph. D. Growth curves for the indicated HCC cells were evaluated by the Trypan blue dye exclusion method (left panel). CCK-8 assays showed that ATGL knockdown inhibited the growth of SK-Hep-1 cells (right panel), however, this effect was completely rescued by treatment with 16 μM DAG+FFA. E. Representative images of the cloning formation assay showed that ATGL knockdown inhibited the growth of SK-Hep-1 cells, however, this effect was completely rescued by treatment with 16 μM DAG+FFA. F. Number of colonies from three experiments were measured, and the results are presented as a bar graph. Data are expressed as mean ± SD of three independent experiments. Statistical significance was concluded at *P < 0.05, **P < 0.01, ***P < 0.001. (TIF 614 kb)