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Additional file 4: of Application of long single-stranded DNA donors in genome editing: generation and validation of mouse mutants

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posted on 21.06.2018 by Gemma Codner, Joffrey MiannĂŠ, Adam Caulder, Jorik Loeffler, Rachel Fell, Ruairidh King, Alasdair Allan, Matthew Mackenzie, Fran Pike, Christopher McCabe, Skevoulla Christou, Sam Joynson, Marie Hutchison, Michelle Stewart, Saumya Kumar, Michelle Simon, Loranne Agius, Quentin Anstee, Kirill Volynski, Dimitri Kullmann, Sara Wells, Lydia Teboul
Figure S3. The figure shows the designs of reagents employed for the generation of conditional alleles. Red triangles mark loxP sites. RNA is transcribed in vitro from a double-stranded DNA template containing the T7 promoter and the donor sequence. The resulting RNA is reverse-transcribed employing a primer that is specific to the donor sequence. Additional sequences (orange boxes, marked as universal) were added to the design for the purpose of facilitating initial screening of animals employing restriction enzyme sites and/or validated primer pairs, with the exception of the Syt7 conditional allele (described in Fig. 1). (PNG 91 kb)


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