13041_2017_317_MOESM4_ESM.pdf (337.58 kB)
Additional file 4: Figure S4. of A T-type channel-calmodulin complex triggers αCaMKII activation
journal contribution
posted on 2017-08-11, 05:00 authored by Hadhimulya Asmara, Ileana Micu, Arsalan Rizwan, Giriraj Sahu, Brett Simms, Fang-Xiong Zhang, Jordan Engbers, Peter Stys, Gerald Zamponi, Ray TurnerBlock of high [K]o-mediated loss of GFP-Cav3.1 - mKate-CaM FRET. Plots of the FRET signal over time in tsA-201 cells coexpressing GFP-Cav3.1 and mKate-CaM. Values are normalized to the mean value of all fluorescence measurements in the time period. a, b. Cells were exposed to Low [K]o (1 mM) or High [K]o (50 mM). A loss of FRET encountered upon exposure to high [K]o is prevented by the Cav3 channel blockers 1 μM mibefradil and 300 μM Ni2+ (a) and in the presence of 0.1 mM BAPTA-AM (b). Average values are mean ± SEM. (PDF 337 kb)
