Additional file 4: Figure S3. of Patient-derived heavy chain antibody targets cell surface HSP90 on breast tumors

Immunoprecipitation of HSP90 from xenograft tumor lysates using HCAb2. RIPA lysates of xenograft tumor pieces were used for immunoprecipitation with 15 μg of HCAb1 and HCAb2. Immunoprecipitated HSP90 protein was detected on an immunoblot using a commercial anti-HSP90 antibody. HCAb2 pulled down HSP90 from 2 h, 6 h and 24 h tumor lysates while HCAb1 did not pull down HSP90. Equal amounts of HCAb1 and HCAb2 were pulled down as detected by anti-mouse IgG antibody. (TIFF 1627 kb)