Additional file 4: Figure S3. of Long-lasting reduction in clonogenic potential of colorectal cancer cells by sequential treatments with 5-azanucleosides and topoisomerase inhibitors

Combinatorial treatments increase CRC cell apoptosis. Annexin V-FITC/PI-double staining of HCT116, DLD-1, and HT-29 cells after sequential treatments with 1 μM 5-aza-dC and 25 μM etoposide (n = 3). Figure 1a shows the treatment scheme. Data are presented as means ± SD. *P < 0.05 compared with DNA demethylating agent treatment group and topoisomerase inhibitor treatment group. (PDF 177 kb)