Additional file 3: Figure S3. of Donepezil suppresses intracellular Ca2+ mobilization through the PI3K pathway in rodent microglia

Sigma 1 receptors were not involved in the donepezil-induced suppression of the TNFα-mediated intracellular Ca2+ elevation in rodent microglial cells. Pretreatment with 5 μM donepezil and 10 μM BD1047, an antagonist of sigma-1 receptors, for 12 h significantly inhibited the elevation of [Ca2+]i induced by TNFα in rat HAPI microglial cells. In this panel, the average trace determined from 5 representative traces of [Ca2+]i in each condition. Dotted line is the average trace of control. (TIFF 222 kb)