Additional file 3: Figure S2. of Improved genome sequencing using an engineered transposase

DNA binding stability of Tn5 vs. Tn5-059. While heating at 74.2 °C is required for Tn5 to dissociate from tagmented DNA to allow following PCR amplification of DNA to reach the level of positive control, the temperature is elevated to 76.6 °C for Tn5-059 to do the same. The negative control is tagmentation without PCR amplification. The positive control is tagmentation followed by complete removal of transposase by Zymo cleaning to allow PCR amplification. (PDF 81 kb)