Additional file 2: Figure S2. of Targeting mitochondrial complex I using BAY 87-2243 reduces melanoma tumor growth
2019-10-24T12:52:13Z (GMT) by
Inhibition of mitochondrial complex I with BAY 87-2243 inhibits OXPHOS and triggers glycolysis in melanoma cells. (A) OCR was measured using polarographic oxygen sensors in a two-chamber Oxygraph (Oroboros) in melanoma cells. BAY 87-2243 was injected in different concentrations (n = 3). (B) SK-MEL-28 and G-361 cells were treated with BAY 87-2243 (10 nM) and the time-dependent production of extracellular lactate was measured (n = 4). (C) OCR was measured using Seahorse analyzer in G-361 cells. BAY 87-2243 was injected (black arrow) in different concentrations followed by consecutive injections of oligomycin (1 μM), FCCP (0.5 μM) and antimycin A (1 μM)/rotenone (1 μM) (n = 6). Data are represented as the mean ± SD. *p < 0.05.